Sr39tk Pet and Suicide Elimination of Human Hscs
نویسندگان
چکیده
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA; Department of Medical and Molecular Pharmacology, University of California, Los Angeles, CA; Department of Medicine Statistics Core; Center for Immunotherapy, Roswell Park Cancer Institute, Buffalo, NY; Department of Medicine, Division of Hematology/Oncology, University of California, Los Angeles, CA; Department of Pediatrics, Division of Hematology/Oncology, Mattel Children’s Hospital, University of California, Los Angeles, CA; Jonsson Comprehensive Cancer Center, Los Angeles, CA, and The Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Los Angeles, CA
منابع مشابه
HSV-sr39TK positron emission tomography and suicide gene elimination of human hematopoietic stem cells and their progeny in humanized mice.
Engineering immunity against cancer by the adoptive transfer of hematopoietic stem cells (HSC) modified to express antigen-specific T-cell receptors (TCR) or chimeric antigen receptors generates a continual supply of effector T cells, potentially providing superior anticancer efficacy compared with the infusion of terminally differentiated T cells. Here, we demonstrate the in vivo generation of...
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We are developing assays for noninvasive, quantitative imaging of reporter genes with positron emission tomography (PET), for application both in animal models and in human gene therapy. We report here a method to improve the detection of lower levels of PET reporter gene expression by utilizing a mutant herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) as a PET reporter gene. The HSV1...
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UNLABELLED Because of its high selectivity and specificity for the imaging reporter probe 9-(4-(18)F-fluoro-3-[hydroxymethyl]butyl)guanine ((18)F-FHBG), the herpes simplex virus type 1 thymidine kinase (HSV1-tk) variant sr39tk is actively being studied as a PET reporter gene. We recently demonstrated the capability of using a prostate-specific transcriptional amplification PET reporter vector, ...
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تاریخ انتشار 2014